Journal: Acta Cirúrgica Brasileira

Article Title: Type I and type III collagen immunoexpression in rabbit skin biopsy samples treated with rosuvastatin gel and autologous platelet-rich plasma

doi: 10.1590/acb402725

Figure Lengend Snippet: Immunohistochemical analysis of the lesions. (a) Immunohistochemical staining for type I collagen on day 17 in the NC and the different treatments. Magnification: 400x. Scale bar: 50 μm. (b) Immunohistochemical staining for type III collagen on day 17 in the NC and the different treatments.

Article Snippet: Antibodies for evaluating collagen immunoexpression in rabbit wounds treated with aPRP, RSV, and aPRP + RSV included: anti-type I collagen primary antibody (1:50, mouse monoclonal, GeneTex, GTX26308), anti-type III collagen primary antibody (1:100, mouse monoclonal, Novus Biologicals, NBP1-05119), and goat anti-mouse IgG H&L (HRP) antibody (1:200, goat, Abcam, ab6789).

Techniques: Immunohistochemical staining, Staining

Journal: Acta Cirúrgica Brasileira

Article Title: Type I and type III collagen immunoexpression in rabbit skin biopsy samples treated with rosuvastatin gel and autologous platelet-rich plasma

doi: 10.1590/acb402725

Figure Lengend Snippet: Plots representing the average percentage of immunostaining for types I and III collagen under different treatments on day 17.

Article Snippet: Antibodies for evaluating collagen immunoexpression in rabbit wounds treated with aPRP, RSV, and aPRP + RSV included: anti-type I collagen primary antibody (1:50, mouse monoclonal, GeneTex, GTX26308), anti-type III collagen primary antibody (1:100, mouse monoclonal, Novus Biologicals, NBP1-05119), and goat anti-mouse IgG H&L (HRP) antibody (1:200, goat, Abcam, ab6789).

Techniques: Immunostaining

Journal: International Journal of Molecular Sciences

Article Title: Anti-Fibrotic Effects of RF Electric Currents

doi: 10.3390/ijms241310986

Figure Lengend Snippet: α-SMA, Col I and Col III expression. ( A ) α-SMA, Col I, and Col III protein expression of myofibroblasts at 48 h of CRET or sham treatment. Fluorescence intensity measurement per MHC channel. Data normalized over the corresponding sham-exposed controls (dashed line represents the control group: 100%). Means ± SEM of the fluorescence intensity/total nuclei of at least three experimental repeats per protein. *: 0.05 > p ≥ 0.01; Student’s t -test. ( B ) Inmunoblot for α-SMA. Representative blots at 48 h of CRET or sham treatment (100 µg protein/lane). GAPDH were used as loading control. C: Control. T: CRET treatment. ( C ) Immunofluorescence expression of α -SMA, Col I, Col III, and merged micrographs. Representative micrographs. Red: α-SMA, Green: Col I or Col III. Blue: cell nuclei. Scale bar: 20 µm.

Article Snippet: At the end of 48 h RF- or sham-exposure, the samples were fixed with 4% paraformaldehyde (Merck, Darmstadt, Germany) and incubated overnight at 4 °C with mouse monoclonal anti- α-smooth muscle actin antibody α-SMA (1:400; cat. no. A 2547; Sigma Aldrich, St. Louis, MO, USA), rabbit polyclonal anti-collagen type I antibody (1:400; cat. no. NB600-408-0.1 mg; Novus; Centennial, CO, USA) and rabbit polyclonal anti-collagen type III alpha 1 antibody (1:400, cat. no. NB600-594SS; Novus; CO, USA).

Techniques: Expressing, Fluorescence, Control, Immunofluorescence

Journal: International Journal of Molecular Sciences

Article Title: Regenerative Skin Remodeling by a Dual Hyaluronic Acid Hybrid Complex in Multimodal Preclinical Models

doi: 10.3390/ijms27021027

Figure Lengend Snippet: Collagen and elastin production in HDFs treated with DHC and other comparators. ( A ) Collagen I, ( B ) Collagen III, and ( C ) Elastin levels were quantified by ELISA. Values are expressed as mean ± SD. * p < 0.05 vs. control; # p < 0.05 between groups.

Article Snippet: Enzyme-Linked Immunosorbent Assay (ELISA) was designed to quantify the production of wrinkle-improving proteins using: Human Pro-Collagen I α1 SimpleStep ELISA Kit (ab210966, Abcam, Cambridge, MA, USA), Human Collagen Type III Alpha 1 ELISA Kit (NBP2-75858, Novus Biologicals, Centennial, CO, USA), Human Elastin ELISA Kit (MBS704171, MyBioSource, San Diego, CA, USA).

Techniques: Enzyme-linked Immunosorbent Assay, Control

Journal: The American Journal of Sports Medicine

Article Title: Mesenchymal Stem Cell Exosome and Fibrin Sealant Composite Enhances Rabbit Anterior Cruciate Ligament Repair

doi: 10.1177/03635465241313142

Figure Lengend Snippet: Ligament-specific matrix expression of ACL repair. (A, B) Immunohistochemical staining for collagen I and collagen III. Representative images of the ACLs achieving the best, median, and worst ACL repair (n = 6). Scale bar: 2 mm. ACL, anterior cruciate ligament.

Article Snippet: Immunohistochemical staining for collagen I (1:1000; Sigma) and collagen III (1:500; Novus Biologicals) was performed with the UltraVision Quanto Detection System (Thermo Fisher Scientific).

Techniques: Expressing, Immunohistochemical staining, Staining